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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...

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Related Experiment Video

Updated: Mar 20, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Lessons from non-canonical splicing.

Christopher R Sibley^1,2, Lorea Blazquez¹, Jernej Ule¹

¹Department of Molecular Neuroscience, UCL Institute of Neurology, Russell Square House, Russell Square, London, WC1B 5EH, UK.

Nature Reviews. Genetics

|June 1, 2016

Summary

This summary is machine-generated.

Recent advances reveal novel non-canonical splicing events in RNA processing. These splicing variations are crucial for gene regulation and evolution but can cause disease, offering new therapeutic targets.

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

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Published on: September 15, 2021

Area of Science:

Molecular Biology
Genetics
Bioinformatics

Background:

Advanced experimental and computational methods provide new insights into transcriptome.
RNA processing includes canonical and non-canonical splicing events.
Non-canonical splicing plays a role in gene expression regulation and evolution.

Purpose of the Study:

To highlight recent discoveries in non-canonical splicing events.
To explain the role of non-canonical splicing in gene expression and evolution.
To discuss the implications of non-canonical splicing in disease and therapeutics.

Main Methods:

Utilizing advanced experimental techniques for transcriptome analysis.
Employing computational approaches to identify novel splicing events.
Reviewing current literature on RNA processing and splicing mechanisms.

Main Results:

Identification of numerous previously unknown non-canonical splicing events.
Cryptic splicing events and unconventional mechanisms are prevalent.
Non-canonical splicing, especially involving transposable elements, contributes to transcript diversity.

Conclusions:

Non-canonical splicing is a significant driver of evolutionary transcript novelty.
Dysregulation of non-canonical splicing can lead to disease.
Understanding non-canonical splicing opens avenues for novel therapeutic strategies.