Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.7K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.7K
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

2.5K
2.5K
Biological Clocks and Seasonal Responses02:45

Biological Clocks and Seasonal Responses

42.0K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
42.0K
Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response01:15

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

449
Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...
449
Sleep-Wake Cycles01:24

Sleep-Wake Cycles

3.2K
Sleep is an essential physiological process vital to maintaining overall well-being. The reticular activating system (RAS), a network of neurons in the brainstem, regulates wakefulness and sleep. While it may seem passive, sleep consists of distinct cycles, each with its unique characteristics and functions. Two key sleep phases are non-rapid eye movement (NREM) and  rapid eye movement (REM).
NREM Sleep
NREM sleep comprises four progressive stages that seamlessly merge:
3.2K
Understanding Sleep01:11

Understanding Sleep

1.8K
Sleep, an essential biological state, involves significant reductions in physical activity, sensory awareness, and interaction with the environment. This complex physiological process is primarily regulated by specific brain regions, notably the hypothalamus and pons, which govern the sleep-wake cycle or circadian rhythm.
The circadian rhythm, a nearly 24-hour cycle, is deeply influenced by environmental light cues. Light exposure directly affects the hypothalamus, which in turn regulates...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trends in circadian rhythms research in fungi since the millenium.

Npj biological timing and sleep·2026
Same author

Roles for Phosphatase PP4 in Rhythmicity and Compensation in the Neurospora Circadian System.

bioRxiv : the preprint server for biology·2026
Same author

A Screen To Identify Protein Phosphatases with Roles in Circadian Period, Temperature Compensation and Output in the <i>Neurospora</i> Circadian Clock.

bioRxiv : the preprint server for biology·2026
Same author

Core clock protein subcellular dynamics coordinate local and global circadian control in syncytia.

The Journal of cell biology·2026
Same author

Rhythmic Nuclear Import Mediated by Importins Regulates the Neurospora Circadian Clock.

Genetics·2026
Same author

The Case for Kinases: A Phosphorylation Driven Model for Circadian Temperature Compensation.

bioRxiv : the preprint server for biology·2026
Same journal

Why microglial repair programs fade.

Cell research·2026
Same journal

Atypical signaling, ligand recognition and selective agonist discovery of complement receptor C5aR2.

Cell research·2026
Same journal

One drug, five targets.

Cell research·2026
Same journal

Smooth emotional response: amygdalar neurovascular coupling drives stress encoding.

Cell research·2026
Same journal

Liquid surrogates of spatial tumor ecosystems.

Cell research·2026
Same journal

MitoCatch directs mitochondria delivery and prevents cell degeneration.

Cell research·2026
See all related articles

Related Experiment Video

Updated: Mar 20, 2026

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
06:53

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

8.9K

Yes, circadian rhythms actually do affect almost everything.

Jay C Dunlap1, Jennifer J Loros1,2

  • 1Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA.

Cell Research
|June 1, 2016
PubMed
Summary
This summary is machine-generated.

Daily fluctuations in intracellular magnesium levels are conserved across species and influence metabolism. The cellular clock controls ion channels, revealing the cell as a comprehensive circadian system.

More Related Videos

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
05:46

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Published on: January 24, 2013

22.3K
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
11:56

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

10.5K

Related Experiment Videos

Last Updated: Mar 20, 2026

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
06:53

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

8.9K
Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
05:46

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Published on: January 24, 2013

22.3K
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
11:56

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

10.5K

Area of Science:

  • Biochemistry
  • Chronobiology
  • Cellular Biology

Background:

  • Circadian rhythms are endogenous biological processes that regulate physiological functions over a 24-hour cycle.
  • Intracellular magnesium (Mg) levels exhibit circadian variations, suggesting a role in metabolic regulation.
  • The precise mechanisms linking circadian clocks to intracellular Mg homeostasis remain incompletely understood.

Purpose of the Study:

  • To investigate the phylogenetic conservation of circadian rhythms in intracellular Mg levels.
  • To explore the functional impact of these Mg rhythms on cellular metabolism.
  • To elucidate the role of the cellular clock in regulating ion channels involved in Mg homeostasis.

Main Methods:

  • Phylogenetic analysis of Mg rhythm conservation.
  • Metabolic assays to assess the impact of Mg fluctuations.
  • Electrophysiological studies of ion channel regulation by the circadian clock.

Main Results:

  • Circadian rhythms of intracellular Mg are conserved across diverse species.
  • These Mg rhythms significantly influence various metabolic pathways.
  • The cellular circadian clock directly regulates ion channels responsible for generating Mg rhythms.

Conclusions:

  • Intracellular Mg levels exhibit a phylogenetically conserved circadian rhythm with broad metabolic implications.
  • The cellular clock functions as an integrated circadian system by regulating ion channels that control Mg homeostasis.
  • Understanding this Mg-based circadian system offers new insights into metabolic regulation and potential therapeutic targets.