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Applications and challenges in using LC-MS/MS assays for quantitative doping analysis.

Zhanliang Wang1, Jianghai Lu2, Yinong Zhang1

  • 1Food & Drug Anti-doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.

Bioanalysis
|June 1, 2016
PubMed
Summary

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is vital for detecting performance-enhancing drugs in athletes. This review covers LC-MS/MS quantification methods for anti-doping analysis, addressing World Anti-Doping Agency regulations.

Keywords:
LC–MS/MSdoping analysisquantification

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Area of Science:

  • Analytical Chemistry
  • Sports Science
  • Biochemistry

Background:

  • Doping in sports poses significant challenges to fair competition.
  • Accurate detection of prohibited substances is crucial for maintaining the integrity of sports.
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful analytical technique for biological sample analysis.

Purpose of the Study:

  • To review and summarize various quantification methods for prohibited substances in athletes.
  • To discuss the application of LC-MS/MS in anti-doping analysis.
  • To evaluate these methods in the context of World Anti-Doping Agency (WADA) regulations.

Main Methods:

  • Literature review of LC-MS/MS-based quantification techniques.
  • Analysis of methods for targeted screening and identification of analytes.
  • Comparison of low-mass resolution and high-resolution/high-accuracy MS approaches.

Main Results:

  • LC-MS/MS assays offer fast and sensitive screening and quantification of targeted doping agents.
  • Both low-mass resolution and high-resolution/high-accuracy MS have distinct advantages and challenges for routine anti-doping analysis.
  • Various quantification strategies exist for detecting prohibited substances according to WADA standards.

Conclusions:

  • LC-MS/MS is an indispensable tool for qualitative and quantitative analysis of doped biological samples in athletes.
  • The choice of LC-MS/MS method depends on the specific analytical requirements for screening, identification, and quantification.
  • Continued development and validation of LC-MS/MS methods are essential for effective anti-doping efforts.