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Intersubunit Concerted Cooperative and cis-Type Mechanisms Modulate Allosteric Gating in Two-Pore-Domain Potassium

Ren-Gong Zhuo1, Peng Peng2, Xiao-Yan Liu1

  • 1State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Key Laboratory of Neuropsychopharmacology, Department of Biochemical Pharmacology, Beijing Institute of Pharmacology and Toxicology Beijing, China.

Frontiers in Cellular Neuroscience
|June 1, 2016
PubMed
Summary

TREK-2 potassium channels regulate excitability and pain. This study reveals how TREK-2 subunit interactions, through cooperative and cis-type mechanisms, control channel gating and responses to 2-APB and pH.

Keywords:
2-APBTREK-2allosteric regulationconcatenated dimerintersubunit interactiontwo-pore domain potassium channel

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Ion Channel Physiology

Background:

  • Two-pore-domain potassium channel TREK-2 is crucial for regulating cellular excitability.
  • TREK-2 dysfunction is implicated in neuropathic pain, mood disorders, and ischemia.
  • The role of subunit interaction in TREK-2 modulation by external stimuli is not fully understood.

Purpose of the Study:

  • To investigate the role of subunit interaction in TREK-2 channel gating.
  • To elucidate the mechanisms by which subunit composition affects TREK-2 function.
  • To understand how allosteric modulations by 2-APB and pH are transduced through TREK-2 subunits.

Main Methods:

  • Constructed TREK-2 subunits with deletions (ΔpCt) or point mutations (G312A) to study subunit stoichiometry.
  • Created concatenated dimers of wild-type (WT) and mutant TREK-2 subunits.
  • Characterized the gating kinetics of these channels in response to 2-Aminoethoxydiphenyl borate (2-APB) and extracellular pH (pHo).

Main Results:

  • Wild-type subunits exhibit dominant and positive effects on mutated subunits, indicating concerted cooperative gating.
  • The WT subunit's influence suggests a cooperative manner in controlling channel gating.
  • Introduction of ΔpCt into specific subunits attenuated sensitivity to 2-APB and pHo, indicating cis-type signal transduction.

Conclusions:

  • TREK-2 channel gating is controlled by intersubunit concerted cooperative mechanisms.
  • Allosteric regulations by 2-APB and pH are modulated via both cooperative and cis-type manners.
  • This study elucidates the subunit-dependent mechanisms underlying TREK-2 pore gating and allosteric regulation.