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Published on: March 9, 2015
Developmental validation of forensic DNA-STR kits: Expressmarker 16+10Y and expressmarker 16+18Y
Huaigu Zhou1, Gang Bi1, Chen Zhang1
1Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China.
Abstract:
DNA-STR analysis is widely used in the forensic science field and obtaining results in shorter time is highly demanded. The developed forensic STR Kit, referred to as Expressmarker 16+10Y (EX16+10Y) and Expressmarker 16+18Y (EX16+18Y), could amplify the common autosomal and Y chromosome STR loci simultaneously. The kits are validated by a series of tests, including DNA mixtures, stutter ratios, PCR based studies, species specificities, inhibitors, sensitivity, sizing precision, reproducibility and parallel tests. The results demonstrated that EX16+10Y and EX16+18Y were useful tools for rapid criminal investigation.
