Related Experiment Video
Updated: Mar 20, 2026
05:05
Technique of Conjunctival Biopsy and Direct Immunofluorescence for Diagnosing Mucous Membrane Pemphigoid
Published on: June 17, 2025
1.5K
Human cervix: an alternative substrate for detecting circulating pemphigus antibodies
Leena Chularojanamontri1, Papapit Tuchinda2, Sumruay Pinkaew1
1Department of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Siriraj, Bangkok Noi, Bangkok, Thailand.
Archives of Dermatological Research
|June 2, 2016
Summary
Human cervix (HC) can serve as a reliable alternative to monkey esophagus (ME) for diagnosing pemphigus vulgaris (PV) using indirect immunofluorescence (IIF). This study confirms HC
Area of Science:
- Immunodermatology
- Diagnostic Pathology
Background:
- Monkey esophagus (ME) is a standard substrate for indirect immunofluorescence (IIF) assays in diagnosing pemphigus vulgaris (PV).
- Ethical concerns and limited availability of ME necessitate alternative substrates for PV diagnosis.
Purpose of the Study:
- To evaluate the efficacy of human cervix (HC) as a viable alternative substrate for diagnosing PV via IIF.
- To compare diagnostic titers between HC and ME substrates.
- To determine the sensitivity and specificity of HC for PV and pemphigus foliaceus (PF) diagnosis.
Main Methods:
- Sera from PV patients were incubated with human cervix (HC) tissues, with varying demographic and clinical characteristics.
- IIF titers on HC were compared to those obtained using monkey esophagus (ME).
- Sensitivity and specificity were assessed using sera from PV, PF, and non-pemphigus patients.
Main Results:
- Demographic factors of HC did not significantly impact IIF titers.
- IIF titers obtained using HC were comparable to those from ME, with 81-100% acceptable values.
- HC demonstrated high sensitivity for PV (90.5%) and PF (71.4%), with an overall specificity of 96.2% for both conditions.
Conclusions:
- Human cervix (HC) is a suitable and effective alternative substrate for diagnosing pemphigus vulgaris (PV) by indirect immunofluorescence (IIF).
- The use of HC can overcome limitations associated with monkey esophagus availability.
- HC offers comparable diagnostic accuracy to ME for PV detection.
Related Concept Videos
01:27Cytomegalovirus Disease
5
Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...
5
01:15Transcytosis of IgG
4.5K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
4.5K