Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Concise Guide to PHARMACOLOGY 2025/26: G protein-coupled receptors.

British journal of pharmacology·2025
Same author

A Tourette Syndrome/ADHD-like Phenotype Results from Postnatal Disruption of CB<sub>1</sub> and CB<sub>2</sub> Receptor Signalling.

International journal of molecular sciences·2025
Same author

High-affinity antibodies specific to the core region of the tau protein exhibit diagnostic and therapeutic potential for Alzheimer's disease.

Alzheimer's research & therapy·2024
Same author

The Effect of Phytocannabinoids and Endocannabinoids on Nrf2 Activity in the Central Nervous System and Periphery.

Neurology international·2024
Same author

Phenothiazines: Nrf2 activation and antioxidant effects.

Journal of biochemical and molecular toxicology·2024
Same author

The Concise Guide to PHARMACOLOGY 2023/24: G protein-coupled receptors.

British journal of pharmacology·2023

Related Experiment Video

Updated: Mar 20, 2026

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
07:41

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

Published on: February 20, 2018

9.6K

The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells.

Maria Grazia Cascio1, Pietro Marini2, Roger G Pertwee3

  • 1School of Medicine, Medical Sciences & Nutrition, Institute of Medical Sciences, University of Aberdeen, Foresterhill, AB252ZD, Scotland, UK. m.cascio@abdn.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|June 2, 2016
PubMed
Summary

This study details a displacement binding assay using radioligands and Chinese Hamster Ovarian (CHO) cells. The assay effectively determines if compounds bind to the human cannabinoid receptor 2 (CB2).

Keywords:
AffinityAgonistAntagonistBindingCannabinoid CB2 receptorsLigandRadioligand

More Related Videos

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
06:56

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

14.6K
High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
10:19

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

Published on: November 23, 2013

16.8K

Related Experiment Videos

Last Updated: Mar 20, 2026

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
07:41

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

Published on: February 20, 2018

9.6K
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
06:56

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

14.6K
High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
10:19

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

Published on: November 23, 2013

16.8K

Area of Science:

  • Pharmacology
  • Biochemistry
  • Cell Biology

Background:

  • Displacement binding assays are crucial for assessing compound affinity to target receptors.
  • These assays are widely used for both plant-derived and synthetic compounds.
  • Understanding receptor binding is fundamental in drug discovery and development.

Purpose of the Study:

  • To describe a specific displacement binding assay protocol.
  • To demonstrate the utility of this assay for the human cannabinoid receptor 2 (CB2).
  • To provide a method for evaluating compound interactions with CB2 receptors.

Main Methods:

  • Utilizing a radioligand in the displacement binding assay.
  • Employing Chinese Hamster Ovarian (CHO) cells.
  • These cells were stably transfected with the human cannabinoid receptor 2 (CB2).

Main Results:

  • The described method allows for the determination of compound binding affinity.
  • Successful application of the assay with CB2 receptors was achieved.
  • The assay is suitable for high-throughput screening of potential CB2 ligands.

Conclusions:

  • The displacement binding assay is a valuable tool for characterizing ligand-receptor interactions.
  • This protocol provides a reliable method for studying the human CB2 receptor.
  • The assay facilitates the identification of compounds with high affinity for CB2.