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Related Experiment Videos

A Functional Assay for GPR55: Envision Protocol.

Sharon Anavi-Goffer1,2, Ruth A Ross3,4

  • 1Department of Behavioral Sciences, Ariel University, Ariel, 40700, Israel. s.goffer@ariel.ac.il.

Methods in Molecular Biology (Clifton, N.J.)
|June 2, 2016
PubMed
Summary

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AlphaScreen® SureFire® assay offers a sensitive method to detect ERK1/2 phosphorylation, a key signaling pathway. This novel technology enhances protein detection compared to traditional ELISA, enabling new target discovery.

Area of Science:

  • Biochemistry
  • Cell Signaling
  • Assay Development

Background:

  • AlphaScreen® SureFire® assay is a sensitive protein detection technology.
  • It utilizes luminescent oxygen channeling, nano-beads, and antibodies.
  • It offers advantages over traditional enzyme-linked immunosorbent assays (ELISA).

Purpose of the Study:

  • To describe a method using AlphaScreen® SureFire® assay.
  • To target ERK1/2 phosphorylation.
  • To investigate GPR55 activation by L-α-lysophosphatidylinositol (LPI) and cannabinoids.

Main Methods:

  • Utilized AlphaScreen® SureFire® assay technology.
  • Quantified protein levels in low sample volumes (<5 μl).
  • Focused on detecting phosphorylated ERK1/2 (pERK1/2).
Keywords:
AlphaLISAAlphaScreenCannabinoid receptorEnspireGPR55High-throughput screeningPhytocannabinoids

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Main Results:

  • Demonstrated the sensitivity of AlphaScreen® SureFire® assay for pERK1/2 detection.
  • Showcased its utility in studying G protein-coupled receptor 55 (GPR55) signaling.
  • Validated the assay for analyzing downstream signaling pathways.

Conclusions:

  • AlphaScreen® SureFire® assay provides a sensitive and efficient method for studying protein phosphorylation.
  • The assay is suitable for investigating signaling pathways like GPR55 activation.
  • This technology enables the detection of novel targets and enhances biological research.