Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Concise Guide to PHARMACOLOGY 2025/26: G protein-coupled receptors.

British journal of pharmacology·2025

Same author

A Tourette Syndrome/ADHD-like Phenotype Results from Postnatal Disruption of CB<sub>1</sub> and CB<sub>2</sub> Receptor Signalling.

International journal of molecular sciences·2025

Same author

High-affinity antibodies specific to the core region of the tau protein exhibit diagnostic and therapeutic potential for Alzheimer's disease.

Alzheimer's research & therapy·2024

Same author

The Effect of Phytocannabinoids and Endocannabinoids on Nrf2 Activity in the Central Nervous System and Periphery.

Neurology international·2024

Same author

Phenothiazines: Nrf2 activation and antioxidant effects.

Journal of biochemical and molecular toxicology·2024

Same author

The Concise Guide to PHARMACOLOGY 2023/24: G protein-coupled receptors.

British journal of pharmacology·2023

Search research articles

Related Experiment Video

Updated: Mar 20, 2026

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

Published on: February 20, 2018

The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells.

Pietro Marini¹, Maria Grazia Cascio², Roger G Pertwee¹

¹School of Medicine, Medical Sciences & Nutrition, Institute of Medical Sciences, University of Aberdeen, AB252ZD, Foresterhill, Scotland, UK.

Methods in Molecular Biology (Clifton, N.J.)

|June 2, 2016

Summary

This study details a cyclic AMP assay for evaluating G-protein-coupled receptor (GPCR) ligand activity. The assay uses readily available kits and Chinese hamster ovarian (CHO) cells expressing the human cannabinoid CB2 receptor.

Keywords:

3-Isobutyl-1-methylxanthine (IBMX)Agonist Antagonist Cannabinoid receptor type 2 Cyclic AMP Forskolin (FSK)Inverse agonist

More Related Videos

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

Published on: January 27, 2014

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Published on: March 22, 2024

Related Experiment Videos

Last Updated: Mar 20, 2026

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

Published on: February 20, 2018

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

Published on: January 27, 2014

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Published on: March 22, 2024

Area of Science:

Pharmacology
Biochemistry
Molecular Biology

Background:

Cyclic AMP (cAMP) assays are crucial for characterizing G-protein-coupled receptor (GPCR) ligand pharmacology.
Understanding ligand interactions with receptors like the human cannabinoid CB2 receptor is vital for drug discovery.

Purpose of the Study:

To describe a practical cyclic AMP assay for assessing GPCR ligand activity.
To demonstrate the utility of the assay using the human cannabinoid CB2 receptor.

Main Methods:

Utilized commercially available, non-radioligand, ready-to-use kits for the cyclic AMP assay.
Employed Chinese hamster ovarian (CHO) cells stably transfected with the human cannabinoid CB2 receptor.

Main Results:

The described cyclic AMP assay effectively measures the pharmacological behavior of ligands.
The assay is suitable for determining agonist, antagonist, and inverse agonist activities at the CB2 receptor.

Conclusions:

This method provides a reliable and accessible approach for functional characterization of GPCR ligands.
The cyclic AMP assay is a valuable tool for research involving the human cannabinoid CB2 receptor and other GPCRs.