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Updated: Mar 20, 2026
A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
Published on: February 20, 2018
The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
Pietro Marini1, Maria Grazia Cascio2, Roger G Pertwee1
1School of Medicine, Medical Sciences & Nutrition, Institute of Medical Sciences, University of Aberdeen, AB252ZD, Foresterhill, Scotland, UK.
Abstract:
The cyclic AMP assay is a functional assay that is commonly used to determine the pharmacological behavior (agonists, antagonists, inverse agonists) of G-protein-coupled receptor (GPCR) ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available non-radioligand ready-to-use kits and Chinese hamster ovarian (CHO) cells stably transfected with the human cannabinoid CB2 receptor.
