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Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
Published on: December 16, 2021
Purification and Adoptive Transfer of Group 3 Gut Innate Lymphoid Cells
Xiaohuan Guo1, Kevin Muite2, Joanna Wroblewska2
1Tsinghua University School of Medicine, Medical Science Building D317, Beijing, 100084, China. guoxiaohuan@biomed.tsinghua.edu.cn.
Abstract:
Recent studies have identified several related but distinct innate lymphoid cells (ILCs) populations that are relatively enriched in the intestinal mucosal and protect the host from various infections. Among ILCs, group 3 ILCs (ILC3s) produce lymphotoxin and IL-22, and play important roles in the development of the immune system and host-bacteria interactions. Here, we describe methods for the isolation and purification of ILC3s from the mouse intestine, and the adoptive transfer of purified ILC3s into recipient mice.

