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Related Experiment Video

Updated: Mar 20, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
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Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

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Sirtuin 6 (SIRT6) Activity Assays.

Minna Rahnasto-Rilla1,2, Maija Lahtela-Kakkonen2, Ruin Moaddel3

  • 1Bioanalytical and Drug Development Unit, National institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD, 21224-6825, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 2, 2016
PubMed
Summary

This study details methods for assaying SIRT6 enzyme activity, focusing on its deacetylation functions. Protocols cover HPLC, magnetic beads, bioaffinity chromatography, fluorogenic, and in silico approaches for robust SIRT6 research.

Keywords:
Activation assaysBioaffinity chromatographyDeacetylation assaysIn silico screeningInhibition assays

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Area of Science:

  • Biochemistry
  • Enzymology
  • Molecular Biology

Background:

  • Sirtuin 6 (SIRT6) exhibits deacetylation, mono-ADP-ribosyltransferase, and deacylation activities.
  • SIRT6 specifically targets histone modifications H3K9Ac and H3K56Ac.

Purpose of the Study:

  • To provide detailed protocols for various SIRT6 activity and inhibition assays.
  • To facilitate reproducible research on SIRT6 enzymatic functions.

Main Methods:

  • High-Performance Liquid Chromatography (HPLC) based activity/inhibition assays.
  • Magnetic beads deacetylation assays.
  • Bioaffinity chromatography assays.
  • Fluorogenic and in silico assays.

Main Results:

  • Detailed protocols for multiple SIRT6 assay methodologies are presented.
  • The described assays enable the study of SIRT6's selective deacetylation of H3K9Ac and H3K56Ac.
  • These methods support the investigation of SIRT6's enzymatic activities and potential inhibitors.

Conclusions:

  • The provided protocols offer a comprehensive toolkit for researchers studying SIRT6.
  • Standardized assay methods are crucial for advancing the understanding of SIRT6's biological roles.
  • These assays will aid in the discovery of novel SIRT6 modulators.