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Updated: Mar 20, 2026

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
Published on: November 30, 2021
Investigation on maternal lineage of a Neolithic group from northern Shaanxi based on ancient DNA
Jing Zhao1, Fang-E Liu2, Song Lin1
1a The Key Laboratory of Biomedical Information Engineering of Ministry of Education , Department of Biological Science and Engineering, School of Life Science and Technology, Xi'an Jiaotong University , Xi'an , PR China.
Abstract:
A magnetic bead purification method was successfully used to extract ancient DNA from the skeletal remains of 10 specimens excavated from Wuzhuangguoliang (Wzhgl) site, which was located in northern Shaanxi. The multidimensional scaling (MDS) and analysis of molecular variance approach (AMOVA) revealed that ancient Wzhgl people bored a very high similarity to southern Han Chinese. By constructing the MJ-network of various modern people including Han Chinese and Japanese, the phylogenetic analysis indicated that the Wzhgl population had close maternal distance with ancient Shandong and Xinjiang people. These findings indicated that Wzhgl contributed to the gene pool of Han Chinese and modern Japanese. In addition, population migration and interflow between Wzhgl people and ancient Shandong or Xinjiang probably occurred in Neolithic period.
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