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Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
Published on: May 28, 2014
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Preparation of positive blood cultures for direct MALDI-ToF MS identification
Andrew M Robinson1, James E Ussher2
1Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
Journal of Microbiological Methods
|June 2, 2016
Abstract:
MALDI-ToF MS can be used to identify microorganisms directly from blood cultures. This study compared two methods of sample preparation. Similar levels of genus- (91% vs 90%) and species-level identifications (79% vs 74%) were obtained with differential centrifugation and SDS methods. The SDS method is faster and requires minimal handling.
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