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A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine
Published on: October 27, 2015
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Intestinal Preparation Techniques for Histological Analysis in the Mouse.
Jonathan M Williams1, Carrie A Duckworth2, Kate Vowell3
1Pathology and Pathogen Biology, Royal Veterinary College, Hatfield, United Kingdom.
Current Protocols in Mouse Biology
|June 2, 2016
Summary
Studying the murine intestinal tract is challenging due to its complex structure. This article presents two novel necropsy sampling methods, gut bundling and Swiss roll, for improved histological analysis and data collection.
Area of Science:
- Veterinary Pathology
- Gastrointestinal Histology
- Murine Models
Background:
- The murine intestinal tract's complex anatomy poses challenges for histological analysis.
- Rapid tissue changes post-sampling hinder high-quality sectioning and quantitative data collection.
Purpose of the Study:
- To describe two standardized necropsy sampling techniques for comprehensive murine intestinal tract histological analysis.
- To facilitate systematic examination and quantitative data acquisition from the entire length of the murine intestine.
Main Methods:
- Gut bundling technique for examining cross-sections (circumferences) of the intestine.
- Adapted Swiss roll technique for obtaining longitudinal tissue sections.
Main Results:
- Both methods enable systematic histological analysis of the entire murine intestinal tract.
- Improved tissue handling and section quality for detailed examination.
- Facilitation of quantitative data collection along the intestinal tract's length.
Conclusions:
- The gut bundling and Swiss roll techniques offer effective solutions for studying murine intestinal histology.
- These methods enhance the ability to perform comprehensive and quantitative analyses of the murine gut.
- Standardized sampling protocols are crucial for reliable gastrointestinal research in murine models.
