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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping
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A Deoxyuridine-Based Far-Red Emitting Viscosity Sensor.

Mengyuan Wang1, Yuanwei Zhang2, Xiling Yue3

  • 1Department of Chemistry, University of Central Florida, P.O. Box 162366, Orlando, FL 32816, USA. wmyround@knights.ucf.edu.

Molecules (Basel, Switzerland)
|June 2, 2016
PubMed
Summary
This summary is machine-generated.

A new fluorescent probe, deoxyuridine (dU) benzothiazolium (BZ) derivative (dU-BZ), shows enhanced fluorescence in high-viscosity environments. This makes dU-BZ a promising tool for microviscosity imaging in biological applications.

Keywords:
Sonogashira couplingbioimagingfar-red fluorescent probemicroviscosity sensormolecular rotornucleosides

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Immunofluorescent Detection of Two Thymidine Analogues CldU and IdU in Primary Tissue
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Area of Science:

  • Organic Chemistry
  • Biophysical Chemistry
  • Materials Science

Background:

  • Traditional UV-excitable fluorophores can cause phototoxicity and autofluorescence interference.
  • Developing probes with long-wavelength absorption is crucial for deep tissue imaging and reduced phototoxicity.
  • Sensing local microviscosity is important for understanding cellular processes.

Purpose of the Study:

  • To synthesize and characterize a novel deoxyuridine (dU) benzothiazolium (BZ) derivative (dU-BZ) for enhanced fluorescence imaging.
  • To investigate the photophysical properties of dU-BZ, particularly its response to changes in viscosity.
  • To evaluate the potential of dU-BZ as a microviscosity probe in biological systems.

Main Methods:

  • Sonogashira coupling reaction for synthesis of the dU-BZ derivative.
  • Spectroscopic analysis (fluorescence intensity, quantum yield, lifetime) in varying viscosity solutions (glycerol/methanol mixtures).
  • Temperature-dependent measurements to understand photophysical behavior.
  • In vitro bioimaging experiments to assess microviscosity sensing capabilities.

Main Results:

  • Successful synthesis of the dU-BZ compound incorporating deoxyuridine for hydrophilicity and a benzothiazolium dye for long-wavelength absorption.
  • A significant 30-fold enhancement in fluorescence intensity was observed with increasing solution viscosity.
  • Fluorescence quantum yields and lifetimes increased with viscosity, aligning with theoretical predictions.
  • dU-BZ demonstrated effective microviscosity sensing in in vitro bioimaging.

Conclusions:

  • The novel dU-BZ derivative exhibits viscosity-sensitive fluorescence, making it suitable as a microviscosity probe.
  • Its long-wavelength absorption and enhanced fluorescence in viscous media offer advantages over traditional fluorophores for bioimaging.
  • dU-BZ holds potential for advanced applications in deep tissue imaging and studying cellular environments.