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Related Experiment Videos

Cell cracking: permeabilizing cells to macromolecular probes.

T F Martin

    Methods in Enzymology
    |January 1, 1989
    PubMed
    Summary
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    A novel ball homogenizer makes cells permeable to large molecules, aiding the study of cellular functions like secretion. This method preserves cell structure for detailed imaging and antibody-based research.

    Area of Science:

    • Cell Biology
    • Biotechnology
    • Molecular Biology

    Background:

    • Studying cellular processes often requires introducing macromolecular probes into cells.
    • Maintaining cellular structural integrity is crucial for understanding functions like secretion.
    • Existing methods may compromise cell morphology, limiting further analysis.

    Purpose of the Study:

    • To develop a method for rendering cells permeable to macromolecular probes.
    • To assess the preservation of cellular morphology after the permeabilization process.
    • To evaluate the utility of this method for functional and structural studies of cells.

    Main Methods:

    • Utilizing a ball homogenizer to treat cells.
    • Assessing cell permeability to macromolecular probes.

    Related Experiment Videos

  • Evaluating cellular morphology using microscopy.
  • Investigating the suitability for immunocytochemistry and immunoneutralization.

    • Main Results:

    • The ball homogenizer successfully renders cells (diameter > 10 microns) permeable to macromolecular probes.
    • Cellular morphology is sufficiently preserved for detailed analysis.
    • The method is compatible with immunocytochemical and immunoneutralization techniques.

    Conclusions:

    • The ball homogenizer provides a viable method for enhancing cell permeability while maintaining structural integrity.
    • This technique offers a valuable tool for investigating cellular functions requiring intact morphology, such as secretion.
    • The method supports advanced applications like immunocytochemistry and immunoneutralization studies.