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Endogenous Mouse Dicer Is an Exclusively Cytoplasmic Protein.

Christian Much1,2, Tania Auchynnikava3, Dinko Pavlinic4

  • 1Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy.

Plos Genetics
|June 3, 2016
PubMed
Summary
This summary is machine-generated.

This study definitively shows that Dicer protein is exclusively found in the cytoplasm of mouse cells, both in adult tissues and during embryonic development. This finding excludes a direct role for Dicer in nuclear RNA processing in mice.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Dicer protein is crucial for microRNA and short-interfering RNA biogenesis.
  • Previous studies suggested Dicer's involvement in nuclear processes like dsRNA clearance in human and mouse cell lines.

Purpose of the Study:

  • To precisely determine the in vivo cellular localization of Dicer in mice.
  • To investigate if Dicer's localization is conserved in mammals.
  • To clarify Dicer's role in nuclear versus cytoplasmic functions.

Main Methods:

  • Utilized an endogenously epitope-tagged Dicer knock-in mouse model.
  • Performed biochemical fractionation and confocal immunofluorescence microscopy on primary mouse cells and tissues.
  • Examined Dicer localization during mouse embryogenesis across all germ layers and extraembryonic tissues.

Main Results:

  • Endogenous Dicer was found to be exclusively cytoplasmic in all examined primary mouse cell lines and adult tissues.
  • No evidence of Dicer shuttling between the nucleus and cytoplasm was observed.
  • FGF or DNA damage signaling did not induce Dicer nuclear translocation.
  • Dicer remained strictly cytoplasmic during all stages of embryogenesis and in all embryonic tissues.

Conclusions:

  • Dicer protein plays an exclusively cytoplasmic role in RNA biogenesis in vivo in mice.
  • The data exclude a direct function for Dicer in nuclear RNA processing in the mouse.
  • Dicer's cytoplasmic localization is a conserved feature in mammalian development and adult tissues.