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Engineering Paper-Based Sensors for Zika Virus.

Robert J Meagher1, Oscar A Negrete1, Koen K Van Rompay2

  • 1Sandia National Laboratories, Biotechnology and Bioengineering Department, Livermore, CA 94551, USA.

Trends in Molecular Medicine
|June 4, 2016
PubMed
Summary

New Zika virus (ZIKV) diagnostic tests are needed. Paper-based sensors with CRISPR/Cas9 offer a rapid, field-ready approach for detecting ZIKV RNA in emerging infectious disease outbreaks.

Keywords:
CRISPR/Cas9emerging infectious diseasesmolecular diagnosticspaper microfluidicstoehold sensors

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Medical Diagnostics

Background:

  • Zika virus (ZIKV) outbreaks in Latin America and Southeast Asia highlight the need for accessible diagnostic tools.
  • Current diagnostic methods may not be suitable for rapid deployment in resource-limited settings.

Purpose of the Study:

  • To present a novel approach for rapid Zika virus RNA detection.
  • To showcase the potential of paper-based sensors combined with CRISPR/Cas9 technology for field diagnostics.

Main Methods:

  • Utilized paper-based sensors for sample analysis.
  • Integrated CRISPR/Cas9 gene-editing technology for specific ZIKV RNA detection.
  • Focused on developing a sensitive and simple detection system.

Main Results:

  • Demonstrated the feasibility of using paper-based sensors for ZIKV RNA detection.
  • Showcased the specificity and sensitivity of the CRISPR/Cas9 system in this context.
  • Highlighted the potential for rapid assay development and deployment.

Conclusions:

  • Paper-based sensors coupled with CRISPR/Cas9 represent a promising strategy for ZIKV diagnostics.
  • This approach facilitates the development of field-ready tools for emerging infectious diseases.
  • Enables rapid response to public health threats posed by viruses like ZIKV.