Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hepatitis01:25

Hepatitis

6
Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver.
6

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Test of CP Symmetry in the Neutral Decays of Λ via J/ψ→ΛΛ[over ¯].

Physical review letters·2026
Same author

Precise Measurement of the Chromoelectric Dipole Moment of the Charm Quark.

Physical review letters·2026
Same author

Precise Measurement of Matter-Antimatter Asymmetry with Entangled Hyperon-Antihyperon Pairs.

Physical review letters·2026
Same author

Observation of Λ[over ¯]p→K^{+}π^{+}π^{-}π^{0} and Λ[over ¯]p→K^{+}π^{+}π^{-}2π^{0}.

Physical review letters·2026
Same author

First Measurement of the D_{s}^{+}→K^{0}μ^{+}ν_{μ} Decay.

Physical review letters·2026
Same author

Observation of the Electromagnetic Radiative Decays of the Λ(1520) and Λ(1690) to γΣ^{0}.

Physical review letters·2026

Related Experiment Video

Updated: Mar 20, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

24.8K

[Tracing hepatitis C virus transmission by using high-throughput sequencing technique].

J N Zhang1, Y K Wang2, Y Jiang

  • 1National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine]
|June 4, 2016
PubMed
Summary

High-throughput sequencing effectively traced hepatitis C virus (HCV) transmission at the quasispecies level. This method identified a transmission link between an HCV-positive individual and a control, but not with a suspected needle-sharing case.

More Related Videos

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
11:14

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

18.9K
Author Spotlight: Advancements and Challenges in Hepatitis B Virus Detection
04:11

Author Spotlight: Advancements and Challenges in Hepatitis B Virus Detection

Published on: December 15, 2023

5.1K

Related Experiment Videos

Last Updated: Mar 20, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

24.8K
Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
11:14

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

18.9K
Author Spotlight: Advancements and Challenges in Hepatitis B Virus Detection
04:11

Author Spotlight: Advancements and Challenges in Hepatitis B Virus Detection

Published on: December 15, 2023

5.1K

Area of Science:

  • Virology
  • Genetics
  • Public Health

Background:

  • Hepatitis C virus (HCV) quasispecies analysis is crucial for understanding transmission dynamics.
  • High-throughput sequencing offers a powerful tool for detailed viral genetic investigations.

Purpose of the Study:

  • To develop and apply a Hiseq high-throughput sequencing approach for analyzing HCV quasispecies.
  • To investigate a potential HCV needle-sharing transmission case within a methadone clinic population.

Main Methods:

  • HCV RNA was extracted, reverse-transcribed to cDNA, and subjected to Hiseq sequencing for quasispecies detection and analysis.
  • Phylogenetic analysis and genetic distance calculations were performed on HCV quasispecies from seroconverted individuals and controls.
  • HCV subtype 3b was identified in the primary case (P1) and selected other individuals.

Main Results:

  • Hiseq sequencing successfully generated millions of unique HCV quasispecies sequences from 9 specimens.
  • Intrapersonal genetic diversity ranged from 0.4% to 12.3%, with interpersonal diversities varying significantly between individuals.
  • Phylogenetic analysis revealed no transmission link between P1 and P2, but confirmed a transmission relationship between P1 and a control individual (C7).

Conclusions:

  • Hiseq high-throughput sequencing is a valuable method for tracing HCV transmission at the quasispecies level.
  • The technique offers high throughput, ease of operation, and cost-effectiveness for epidemiological studies.
  • This study successfully differentiated transmission links, highlighting the utility of quasispecies analysis in public health investigations.