Related Experiment Video
Updated: Mar 20, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
High-quality genome sequence and description of Bacillus ndiopicus strain FF3(T) sp. nov
C I Lo1, R Padhmanabhan2, O Mediannikov1
1Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, Marseille, France; Campus International UCAD-IRD, Dakar, Senegal.
Abstract:
Strain FF3(T) was isolated from the skin-flora of a 39-year-old healthy Senegalese man. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry did not allow any identification. This strain exhibited a 16S rRNA sequence similarity of 96.8% with Bacillus massiliensis, the phylogenetically closest species with standing nomenclature. Using a polyphasic study made of phenotypic and genomic analyses, strain FF3(T) was Gram-positive, aeroanaerobic and rod shaped and exhibited a genome of 4 068 720 bp with a G+C content of 37.03% that coded 3982 protein-coding and 67 RNA genes (including four rRNA operons). On the basis of these data, we propose the creation of Bacillus ndiopicus sp. nov.
Related Concept Videos
Bacterial Phylum Firmicutes
DNA Bacteriophages
Modern Molecular Taxonomy
Applications of Molecular Taxonomy
Bacterial Phylum Actinobacteria
Cytoskeletal Proteins in Bacteria

