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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Hybridization-based aptamer labeling using complementary oligonucleotide platform for PET and optical imaging.

Jun Young Park1, Tae Sup Lee2, In Ho Song2

  • 1Department of Nuclear Medicine, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea; Department of Anatomy, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju 26426, Republic of Korea.

Biomaterials
|June 4, 2016
PubMed
Summary

This study introduces a novel aptamer conjugation platform for molecular imaging. The platform enhances aptamer stability and targeting of cancer cells, showing great potential for theranosis applications.

Keywords:
AptamerHybridizationMolecular imagingOligonucleotidePETRadioisotope

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Medical Imaging

Background:

  • Aptamers are synthetic nucleic acid ligands with significant potential in molecular imaging and theranosis.
  • Current methods for aptamer application require efficient conjugation strategies for optimal performance.

Purpose of the Study:

  • To develop a novel aptamer conjugation platform based on complementary oligonucleotide (cODN) hybridization.
  • To evaluate the utility of this platform for aptamer-based molecular imaging agents.

Main Methods:

  • A cODN hybridization-based aptamer conjugation platform was designed.
  • The cODN was pre-labeled with fluorescent dyes or radioisotopes.
  • Aptamers were hybridized with matched cODN sequences in aqueous conditions.
  • In vitro and in vivo studies were conducted to assess serum stability and target binding affinity.

Main Results:

  • The cODN platform-hybridized aptamers demonstrated enhanced serum stability.
  • Specific binding affinity towards target cancer cells was observed both in vitro and in vivo.
  • The platform facilitated the use of aptamers as effective molecular imaging agents.

Conclusions:

  • The developed cODN hybridization platform provides a versatile method for aptamer conjugation.
  • This platform shows significant promise for advancing aptamer-based molecular imaging and theranosis.
  • The strategy enhances the stability and targeting capabilities of aptamers for biomedical applications.