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Published on: October 23, 2011
mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification
Joanna Jakubowska1, Agnieszka Maciejewska2, Ryszard Pawłowski2
1Department of Forensic Medicine, Laboratory of Forensic Biology and Genetics, Medical University of Gdansk, ul.Dębowa 23, Gdańsk, 80-204, Poland. jjakubowska@gumed.edu.pl.
Abstract:
Recently mRNA profiling has been widely proposed as a universal tool for biological fluids identification. Here, we describe a test for vaginal fluid identification that combines detection of five markers: vaginal mRNAs and Lactobacilli in end-point PCR reaction. The test detects the following transcripts: HBD1 (Human beta-defensin 1), MUC4 (Mucin 4), MMP11 (Matrix metalloproteinase 11), housekeeping gene G6PDH (glucose 6-phosphate dehydrogenase), and the 16S-23S rRNA intergenic spacer regions of L. crispatus and L. gasseri/L. johnsonii. Simultaneous analysis of five vaginal markers and a housekeeping gene ensures high specificity and reliability in the detection of vaginal material, which could not be obtained using detection of a single marker.
