Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Venous Thrombosis III: Interprofessional Care01:29

Venous Thrombosis III: Interprofessional Care

456
Venous thrombosis requires effective prevention and treatment strategies to improve patient outcomes and reduce potential complications.Prevention StrategiesHealthcare providers must prioritize preventing venous thromboembolism (VTE) for all adult patients upon admission. Interventions depend on bleeding and thrombosis risk, medical history, current medications, diagnoses, planned procedures, and patient preferences. Patients on bed rest should change positions every two hours and, if not...
456
Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies01:20

Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies

422
The key difference between Superficial Vein Thrombosis (SVT) and Deep Vein Thrombosis (DVT) lies in their location and severity.Clinical ManifestationsSVT typically presents with localized pain, tenderness, and redness along the course of a superficial vein, often accompanied by a palpable, cord-like structure under the skin. This condition is usually less dangerous than DVT but can be uncomfortable and may lead to complications such as cellulitis or, rarely, a clot extension into the deep...
422
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

2.3K
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Staphylococcus</i> species and <i>Mammaliicoccus</i> species isolated from milk and teat apex of Lacaune sheep: Occurrence of antimicrobial resistance.

JDS communications·2026
Same author

CRISPR-based mutagenesis of lipopolysaccharide biosynthesis genes in Leptospira interrogans reveals gene essentiality and confirms the role of an O-antigen polymerase.

Scientific reports·2026
Same author

Functional Validation of the Proteome-Identified LIC_13056 Putative Lipoprotein of <i>Leptospira interrogans</i> and the Potential Role in Pathogenesis.

International journal of molecular sciences·2026
Same author

The role of leptospiral proteins in immune evasion and inflammatory response stimulation in HEK293T cell monolayers.

Frontiers in immunology·2025
Same author

Bull urine and semen as potential vectors of disease transmission for Leptospira borgpetersenii serovar Hardjo.

Journal of dairy science·2025
Same author

Revisiting Host-Binding Properties of LigA and LigB Recombinant Domains.

Microorganisms·2025

Related Experiment Video

Updated: Mar 20, 2026

Author Spotlight: Leptospira DNA Detection in Water for Environmental Analysis and Disease Surveillance
06:05

Author Spotlight: Leptospira DNA Detection in Water for Environmental Analysis and Disease Surveillance

Published on: June 14, 2024

2.0K

Decrease in antithrombin III and prothrombin serum levels contribute to coagulation disorders during leptospirosis.

Luis G V Fernandes1,2, Antonio F S Filho3, Gisele O Souza3

  • 1Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900, Sao Paulo, SP, Brazil.

Microbiology (Reading, England)
|June 5, 2016
PubMed
Summary

Leptospira bacteria cause leptospirosis, leading to severe hemorrhage. This study suggests hemorrhage results from stimulated coagulation and overconsumption of clotting factors, not direct bacterial action on thrombin inhibitors.

More Related Videos

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
04:25

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach

Published on: August 8, 2025

1.4K
A Modular Workflow for Quantitative, Structural and Functional Analysis of Leptospira Biofilms
08:51

A Modular Workflow for Quantitative, Structural and Functional Analysis of Leptospira Biofilms

Published on: December 19, 2025

292

Related Experiment Videos

Last Updated: Mar 20, 2026

Author Spotlight: Leptospira DNA Detection in Water for Environmental Analysis and Disease Surveillance
06:05

Author Spotlight: Leptospira DNA Detection in Water for Environmental Analysis and Disease Surveillance

Published on: June 14, 2024

2.0K
Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
04:25

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach

Published on: August 8, 2025

1.4K
A Modular Workflow for Quantitative, Structural and Functional Analysis of Leptospira Biofilms
08:51

A Modular Workflow for Quantitative, Structural and Functional Analysis of Leptospira Biofilms

Published on: December 19, 2025

292

Area of Science:

  • Microbiology
  • Pathogenesis
  • Infectious Diseases

Background:

  • Leptospirosis, caused by Leptospira bacteria, is a global zoonotic disease with severe manifestations including hemorrhage.
  • The precise mechanisms underlying hemorrhage in leptospirosis remain poorly understood.

Purpose of the Study:

  • To investigate the interaction between Leptospira and key coagulation factors.
  • To elucidate the role of coagulation abnormalities in the pathogenesis of leptospirosis-associated hemorrhage.

Main Methods:

  • Analysis of antithrombin III and prothrombin levels in human serum samples from leptospirosis patients.
  • Measurement of prothrombin fragment F1+2 as a marker of coagulation activation.
  • Experimental infection of hamsters with virulent Leptospira strains to correlate hemorrhage with coagulation factor levels.

Main Results:

  • Leptospira strains did not bind or degrade antithrombin III, despite its reduced levels in convalescent sera.
  • Lower prothrombin levels were observed in patients during acute and convalescent phases compared to healthy individuals.
  • Increased prothrombin fragment F1+2 indicated stimulated coagulation, correlating with hemorrhage in infected hamsters and decreased thrombin inhibitors/prothrombin.

Conclusions:

  • Hemorrhage in leptospirosis appears to be an indirect consequence of stimulated coagulation and subsequent overconsumption of coagulation factors.
  • Leptospira pathogenesis may involve a complex interplay with the host's hemostatic system, leading to bleeding and organ failure.