Related Experiment Video

Updated: Mar 20, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

Silymarin suppressed lung cancer growth in mice via inhibiting myeloid-derived suppressor cells

Tiancong Wu1, Wen Liu2, Wenjie Guo2

  • 1Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, Jiangsu Province, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|June 5, 2016
PubMed

Insights

Silymarin demonstrated significant antitumor effects against Lewis lung cancer (LLC) xenografts in mice. It suppressed tumor growth by enhancing CD8(+) T cell activity and reducing myeloid-derived suppressor cells (MDSC).

Area of Science:

  • Immunology
  • Cancer Research
  • Pharmacology

Background:

  • Lewis lung cancer (LLC) is a significant challenge in oncology.
  • Understanding the immune microenvironment's role in tumor progression is crucial.
  • Natural compounds like Silymarin are being explored for their therapeutic potential.

Purpose of the Study:

  • To investigate the antitumor activity of Silymarin in a mouse model of colon cancer xenograft using Lewis lung cancer (LLC) cells.
  • To elucidate the mechanisms underlying Silymarin's effects on the tumor microenvironment and immune response.

Main Methods:

  • Establishment of a Lewis lung cancer (LLC) xenograft mouse model.
  • Administration of Silymarin at doses of 25 and 50 mg/kg.
  • Assessment of tumor growth, apoptosis, immune cell infiltration (CD8(+) T cells), cytokine levels (IL-10, IL-2, IFN-γ), and myeloid-derived suppressor cells (MDSC) markers (iNOS2, Arg-1, MMP9).

Main Results:

  • Silymarin significantly suppressed LLC xenograft tumor growth and induced apoptosis.
  • Silymarin enhanced CD8(+) T cell infiltration and function, increased IL-2 and IFN-γ levels, and decreased IL-10 levels.
  • Silymarin reduced the proportion and function of myeloid-derived suppressor cells (MDSC) by downregulating iNOS2, Arg-1, and MMP9 mRNA expression.

Conclusions:

  • Silymarin exhibits potent antitumor activity against LLC xenografts.
  • The mechanism involves the inhibition of MDSC and promotion of CD8(+) T cell-mediated anti-tumor immunity.
  • These findings support the potential of Silymarin as a therapeutic agent for lung cancer.
Keywords:
CD8+ T cellLung cancerMDSCSilymarin

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.8K
Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.9K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.5K
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.7K