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Updated: Mar 20, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
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Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

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Involvement of α5 integrin in survivin-mediated osteosarcoma metastasis

Xiao-Zhou Liu1, Cheng-Jun Li2, Su-Jia Wu2

  • 1Southern Medical University, Guangzhou 510515, China; Department of Orthopedics, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.

Asian Pacific Journal of Tropical Medicine
|June 5, 2016
PubMed
Abstract

Insights

Survivin knockdown inhibits osteosarcoma (bone cancer) cell invasion and migration by reducing α5 integrin. This suggests survivin-targeting therapies may effectively treat osteosarcoma metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma is a primary bone cancer with a high risk of metastasis.
  • Survivin is a protein implicated in cancer cell proliferation, apoptosis, and angiogenesis.
  • The role of survivin in osteosarcoma metastasis requires further elucidation.

Purpose of the Study:

  • To investigate the role of survivin in osteosarcoma metastasis.
  • To determine the relationship between survivin and α5 integrin expression in osteosarcoma cells.
  • To evaluate the therapeutic potential of targeting survivin in osteosarcoma.

Main Methods:

  • Small interfering RNA (siRNA) was used to knockdown survivin and α5 integrin expression in MG63 osteosarcoma cells.
  • Western blotting, immunostaining, flow cytometry, and fluorescence microscopy assessed protein expression.
  • In vitro assays (Transwell, wound healing) and in vivo studies in nude mice evaluated cell invasion, migration, and tumor growth.

Main Results:

  • Survivin knockdown significantly inhibited osteosarcoma cell invasion and migration in vitro.
  • Survivin knockdown reduced the expression of α5 integrin on the cell surface.
  • Targeting α5 integrin with an antibody also decreased cell invasion and migration.
  • In vivo, survivin knockdown slowed tumor growth in nude mice.

Conclusions:

  • Survivin plays a crucial role in promoting osteosarcoma cell invasion and migration, partly through the regulation of α5 integrin.
  • Targeting survivin represents a promising therapeutic strategy for controlling osteosarcoma metastasis.
Keywords:
MetastasisOsteosarcomaSurvivinα5 integrin

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