Related Experiment Video
Updated: Mar 20, 2026

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface
Published on: July 4, 2018
Subgingival microbiota in individuals with severe chronic periodontitis
Chi-Ying Tsai1, Chuan Yi Tang2, Te-Sheng Tan3
1Department of Oral Maxillofacial Surgery, Chang Gung Memorial Hospital, Tao-Yuan County, Taiwan.
Background/Purpose:
Subgingival microorganisms are potentially associated with periodontal diseases. However, the correlation between the variance in the periodontal microbiome and the prevalence and severity of periodontitis remains unclear. The aim of this study was to determine the subgingival microbiota in Taiwanese individuals with severe chronic periodontitis (SP).
Methods:
The composition of the subgingival microbiota in healthy and diseased individuals was compared using a 16S rRNA metagenomic approach and quantitative polymerase chain reaction (qPCR). A total of 20 samples, including 10 from healthy individuals and 10 from SP patients, were analyzed.
Results:
We found high microbial diversity, with an average of 774 classified phylotypes per sample and a total of six bacterial phyla across all samples. Cluster analysis by principal component analysis and heat map showed that the bacterial communities were different in the two groups. Streptococcus dominated across all the healthy samples, whereas Prevotella, Porphyromonas, and Treponema were highly abundant across all diseased samples. At least 13 bacterial genera were conserved among all the samples. Only eight genera, including Lautropia, Parvimonas, Actinomyces, Capnocytophaga, Paludibacter, Streptococcus, Haemophilus, and Corynebacterium, were significantly enriched in the healthy group, and six genera, including Porphyromonas, Treponema, Tannerella, Aggregatibacter, Peptostreptococcus, and Filifactor, were significantly enriched in the diseased group. Furthermore, a trend of abundance of bacteria at the species level measured by qPCR in all samples was consistent with the 16S rRNA metagenomics results.
Conclusion:
This study is the first in Taiwan to provide a picture of the microbiome in SP via 16S rRNA metagenomics.
More Related Videos
Related Concept Videos
The Oral Microbiota
Microbiota of the Respiratory Tract
Development of the Oral Microbiota
Microbiota of the Stomach and Small Intestine
The Skin Microbiota
Microbiota of the Urogenital Tract

