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Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry LA-ICP-MS
Published on: January 22, 2017
Direct imaging of elemental distributions in tissue sections by laser ablation mass spectrometry
Mohammadreza Shariatgorji1, Anna Nilsson1, Maximilian Bonta2
1Biomolecular Imaging and Proteomics, Dept. of Pharmaceutical Biosciences, Uppsala University, Box 591 BMC, SE-75124 Uppsala, Sweden.
Abstract:
We present a strategy for imaging of elements in biological tissues using laser ablation (LA) mass spectrometry (MS), which was compared to laser ablation inductively coupled plasma (LA-ICP) MS. Both methods were adopted for quantitative imaging of elements in mouse kidney, as well as traumatic brain injury model tissue sections. MS imaging (MSI) employing LA provides quantitative data by comparing signal abundances of sodium from tissues to those obtained by imaging quantitation calibration standards of the target element applied to adjacent control tissue sections. LA-ICP MSI provided quantitative data for several essential elements in both brain and kidney tissue sections using a dried-droplet approach. Both methods were used to image a rat model of traumatic brain injury, revealing accumulations of sodium and calcium in the impact area and its peripheral regions. LA MSI is shown to be a viable option for quantitative imaging of specific elements in biological tissue sections.
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