Related Experiment Video
Updated: Mar 20, 2026
IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
Published on: November 13, 2023
[3D Collagen Hydrogel Culture of Rat Calvarial Osteoblasts]
Objective:
To establish a collagen hydrogel three-dimensional culture model with rat calvarial osteoblasts (ROBs).
Methods:
ROBs were obtained through enzyme digestion of segregated neonatal SD rat skull. The collagen hydrogel three-dimensional culture model was established by mixing ROBs with different concentrations of type I rat tail collagen (collagen concentration of 1, 2, 3 mg/mL), DMEM medium and NaOH under adjusted PH and a temperature of 37 degrees C. Cell viability and activity were detected by FDA/PI staining and CCK-8 3 d after cell culture. The optimal culture method of 3D collagen hydrogel was identified. Cell distribution was observed using scanning electron microscopy and HE staining.
Results:
ROBs collagen was formed firmly at 2 mg/mL, which had significantly higher levels of cell viability and activity than those at 1 mg/mL and 3 mg/mL. Scanning electron microscopy and HE staining showed that cells under the 2 mg/mL collagen culture system adhered with collagen tightly and distributed homogeneously.
Conclusion:
A collagen hydrogel 3D culture model was established successfully by mixing ROBs with collagen at 2 mg/mL.
