Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Tensor, Vector, and Scalar Polarizations in the Stochastic Gravitational-Wave Background.

Physical review letters·2018
Same author

Prospects for observing and localizing gravitational-wave transients with Advanced LIGO, Advanced Virgo and KAGRA.

Living reviews in relativity·2018
Same author

Effects of early comprehensive interventions on child neurodevelopment in poor rural areas of China: a moderated mediation analysis.

Public health·2018
Same author

D-Meson Azimuthal Anisotropy in Midcentral Pb-Pb Collisions at sqrt[s]_{NN}=5.02  TeV.

Physical review letters·2018
Same author

GW170817: Implications for the Stochastic Gravitational-Wave Background from Compact Binary Coalescences.

Physical review letters·2018
Same author

Increased circulating microparticles in women with preeclampsia.

International journal of laboratory hematology·2018

Related Experiment Video

Updated: Mar 20, 2026

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle
08:11

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Published on: March 31, 2013

15.3K

Analysis of cilia structure and function in zebrafish.

E Leventea1, K Hazime1, C Zhao2

  • 1The University of Sheffield, Sheffield, United Kingdom.

Methods in Cell Biology
|June 7, 2016
PubMed
Summary

Cilia, essential for sensory functions and development, are microtubule-based cell structures. Zebrafish models aid in studying cilia and ciliopathies, offering protocols for examining cilia defects.

Keywords:
CiliopathyHair cellLeft–right asymmetryOlfactionPhotoreceptorPronephros

More Related Videos

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
09:33

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Published on: November 18, 2017

8.8K
Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
08:42

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Published on: February 19, 2016

8.5K

Related Experiment Videos

Last Updated: Mar 20, 2026

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle
08:11

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Published on: March 31, 2013

15.3K
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
09:33

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Published on: November 18, 2017

8.8K
Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
08:42

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Published on: February 19, 2016

8.5K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genetics

Background:

  • Cilia are microtubule-based cell protrusions vital for sensory perception (light, odor) and embryonic development (asymmetry, morphogenesis).
  • They are implicated in mechanosensation and are found across most vertebrate organs.
  • Human cilia abnormalities, termed ciliopathies, have significant health implications.

Purpose of the Study:

  • To provide protocols for examining cilia morphology, motility, and defects.
  • To highlight the utility of zebrafish as a model organism for studying cilia and ciliopathies.
  • To focus on cilia examination during embryonic and early postembryonic development.

Main Methods:

  • Utilizing zebrafish for genetic analysis and advanced imaging of cilia.
  • Employing forward and reverse genetic strategies to generate cilia mutants.
  • Developing and applying protocols to assess cilia in various organs.

Main Results:

  • Zebrafish models exhibit defects mirroring human ciliopathies.
  • Genetic and imaging approaches enable detailed study of cilia function and dysfunction.
  • Protocols allow for comprehensive examination of cilia morphology and motility.

Conclusions:

  • Zebrafish are a powerful model for understanding cilia biology and ciliopathies.
  • The provided protocols facilitate the study of cilia-related defects in developmental contexts.
  • This work aids in advancing research into human cilia disorders.