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Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

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Published on: April 21, 2023

Silver-Lactoferrin Nanocomplexes as a Potent Antimicrobial Agent.

Paweł Pomastowski¹, Myroslav Sprynskyy¹, Petar Žuvela²

¹Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Interdisciplinary Centre for Modern Technologies, Nicolaus Copernicus University , 87-100 Toruń, Poland.

Journal of the American Chemical Society

|June 7, 2016

Summary

Silver immobilization into bovine lactoferrin (LTF) involves a two-stage heterogeneous process, resulting in complexes with confirmed antibacterial activity against clinical bacteria.

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Area of Science:

Biomaterials Science
Nanotechnology
Microbiology

Background:

Bovine lactoferrin (LTF) is a promising biomaterial for drug delivery.
Silver nanoparticles exhibit potent antimicrobial properties.
Developing effective silver-LTF complexes requires understanding immobilization mechanisms.

Purpose of the Study:

Investigate silver immobilization onto/into LTF.
Characterize physicochemical properties of silver-LTF complexes.
Evaluate the antibacterial activity of Ag-LTF complexes.

Main Methods:

Batch sorption kinetics, MALDI-TOF/TOF-MS, ICP-MS, FTIR, SERS, TEM, I-DE, zeta potential measurements.
Molecular dynamics (MD) and density functional theory (DFT) simulations.
Flow cytometry and antibiogram assays for antimicrobial evaluation.

Main Results:

Silver immobilization into LTF follows a heterogeneous two-stage process: external sorption and internal diffusion/binding.
Spectroscopic and microscopic analyses confirmed silver binding within the LTF structure.
Simulations elucidated the binding mechanism and supported silver ion reduction.
Synthesized Ag-LTF complexes demonstrated significant antimicrobial activity against clinical bacteria.

Conclusions:

The study elucidates the mechanism of silver immobilization in LTF.
Ag-LTF complexes exhibit validated antimicrobial properties.
This research supports the development of novel antimicrobial biomaterials.