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Detection of emerging rotavirus G12P[8] in Sonora, México

G González-Ochoa, G de J, P M Calleja-García

    Acta Virologica
    |June 7, 2016
    PubMed

    Insights

    Rotavirus gastroenteritis remains common in young children. This study identified the emerging G12P[8] rotavirus genotype in Mexican children, highlighting the need for ongoing vaccine effectiveness surveillance.

    Area of Science:

    • Virology
    • Pediatrics
    • Public Health

    Background:

    • Rotavirus is a leading cause of severe gastroenteritis in children globally.
    • The introduction of rotavirus vaccines necessitates ongoing monitoring of circulating viral strains.
    • Understanding rotavirus genotype prevalence is crucial for assessing vaccine efficacy.

    Purpose of the Study:

    • To determine the genotypes of rotavirus strains in children with gastroenteritis in Mexico post-vaccine introduction.
    • To analyze the phylogenetic relationships of identified rotavirus strains.
    • To report the presence of the emerging G12P[8] rotavirus genotype in Mexico.

    Main Methods:

    • Fecal samples from children with gastroenteritis were tested for rotavirus using a commercial kit.
    • Viral RNA was extracted, and VP4 and VP7 genes were amplified via RT-PCR.
    • Gene sequencing, cloning, and phylogenetic analysis were performed to characterize rotavirus strains.

    Main Results:

    • Rotavirus was detected in 14% of samples.
    • The G12P[8] genotype was identified in 28.5% of rotavirus-positive samples.
    • Phylogenetic analysis placed G12 genotypes in lineage III and P[8] in lineage V, distinct from earlier Mexican strains.

    Conclusions:

    • The G12P[8] rotavirus strain, recognized globally as emerging, was detected for the first time in Mexico.
    • Continuous surveillance is recommended to track rotavirus genotype distribution and evaluate current rotavirus vaccine suitability.
    Keywords:
    rotavirus; gastroenteritis; G12P[8].

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