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Updated: Mar 19, 2026

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development
Published on: June 16, 2022
Formation of the inner ear during embryonic and larval development of the cichlid fish (Oreochromis mossambicus)
Jochen Weigele1,2, Tamara A Franz-Odendaal2, Reinhard Hilbig1
1a Zoological Institute , University of Stuttgart-Hohenheim , Stuttgart , Germany.
Background:
The vertebrate inner ear comprises mineralized elements, namely the otoliths (fishes) or the otoconia (mammals). These elements serve vestibular and auditory functions. The formation of otoconia and otoliths is described as a stepwise process, and in fish, it is generally divided into an aggregation of the otolith primordia from precursor particles and then a growth process that continues throughout life.
Results:
This study was undertaken to investigate the complex transition between these two steps. Therefore, we investigated the developmental profiles of several inner ear structural and calcium-binding proteins during the complete embryonic and larval development of the cichlid fish Oreochromis mossambicus in parallel with the morphology of inner ear and especially otoliths. We show that the formation of otoliths is a highly regulated temporal and spatial process which takes place throughout embryonic and larval development.
Conclusions:
Based on our data we defined eight phases of otolith differentiation from the primordia to the mature otolith.
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