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iTRAQ technology-based identification of human peripheral serum proteins associated with depression
1Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University-Daqing, Daqing, Heilongjiang 163000, PR China.
Neuroscience
|June 9, 2016
Summary
Researchers identified key serum proteins that are significantly elevated in patients with clinical depression. These proteins, including CRP, ITIH4, SAA1, and ANGPTL3, show potential as novel biomarkers for diagnosing depression.
Area of Science:
- Proteomics
- Biomarker Discovery
- Psychiatric Disorders
Background:
- Clinical depression is a widespread and severe psychiatric disorder linked to disability and suicide.
- Serum proteins may offer potential biomarkers for depression diagnosis.
Purpose of the Study:
- To identify differentially expressed serum proteins in patients with clinical depression.
- To validate potential protein biomarkers for depression diagnosis.
Main Methods:
- Pooled serum samples from depressed patients and healthy controls were analyzed using isobaric tag for relative and absolute quantitation (iTRAQ) and tandem mass spectrometry.
- Ingenuity pathway analysis (IPA) was used for pathway network identification.
- Enzyme-linked immunosorbent assay (ELISA) validated four key proteins.
Main Results:
- A total of 472 proteins were identified, with 154 showing differential abundance between groups.
- Levels of C-reactive protein (CRP), Inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), Serum amyloid A1 (SAA1), and Angiopoietin-like 3 (ANGPTL3) were significantly increased in depressed patients.
- These proteins were identified as potential diagnostic markers.
Conclusions:
- Elevated serum levels of CRP, ITIH4, SAA1, and ANGPTL3 suggest their potential as diagnostic biomarkers for clinical depression.
- Further research into these proteins could lead to improved diagnostic tools for depression.
