Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Choice of surgical treatment tactics in a comorbid patient with hemodynamically significant coronary and cerebrovascular lesions in combination with posttraumatic arteriovenous fistula of the brachial artery].

Angiologiia i sosudistaia khirurgiia = Angiology and vascular surgery·2026
Same author

[Late surgical conversions after endoprosthetic repair of abdominal aortic aneurysms].

Angiologiia i sosudistaia khirurgiia = Angiology and vascular surgery·2026
Same author

[A clinical example of successful stent graft implantation with an additional bridge graft in a patient with aortic damage].

Khirurgiia·2025
Same author

Semi-empirical approach for evaluating the cooling performance of ytterbium-doped crystals.

Applied optics·2025
Same author

Delivery of micro- and nanoparticles from solutions into a linear quadrupole trap using the paper spray method.

European journal of mass spectrometry (Chichester, England)·2025
Same author

Evaluation of anti-HIV-1 (Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus type 1) activity of 6HP and 3TC in vitro using MT-4 cell line variants with different replicative activity.

Voprosy virusologii·2024

Related Experiment Video

Updated: Mar 19, 2026

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
12:57

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Published on: November 16, 2017

8.7K

Application of Laser Correlation Spectroscopy for Measuring Virus Size.

V N Nikiforov1, S E Vinogradov2, A V Ivanov2

  • 1Physical Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia. nvn@lt.phys.msu.ru.

Bulletin of Experimental Biology and Medicine
|June 9, 2016
PubMed
Summary
This summary is machine-generated.

Dynamic light scattering accurately measured virus sizes. Human immunodeficiency viruses (HIV) measured 104 nm, and hepatitis A viruses (HAV) measured 28 nm, matching electron microscopy results.

Keywords:
HIV-1electron microscopyhepatitis A viruslaser correlation spectroscopy (dynamic light scattering)virus size

More Related Videos

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
08:31

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

11.6K
Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
14:59

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

13.5K

Related Experiment Videos

Last Updated: Mar 19, 2026

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
12:57

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Published on: November 16, 2017

8.7K
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
08:31

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

11.6K
Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
14:59

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

13.5K

Area of Science:

  • Virology
  • Biophysics

Background:

  • Accurate sizing of viruses is crucial for understanding their biology and for developing diagnostics and therapeutics.
  • Traditional methods like electron microscopy require sample fixation, which can alter virus morphology.

Purpose of the Study:

  • To evaluate the efficacy of Dynamic Light Scattering (DLS) for determining the size of intact human immunodeficiency viruses (HIV) and hepatitis A viruses (HAV).
  • To compare DLS measurements with established electron microscopy data.

Main Methods:

  • Dynamic Light Scattering (DLS), also known as laser correlation spectroscopy, was employed.
  • Correlation functions of light scattered by purified HIV-1 (subtypes A and B) and HAV samples were measured.
  • Results were compared with data obtained from electron microscopy of fixed virus samples.

Main Results:

  • DLS determined the size of HIV-1 virions to be 104 nm.
  • DLS determined the size of HAV virions to be 28 nm.
  • The sizes obtained via DLS showed good agreement with those determined by electron microscopy.

Conclusions:

  • Dynamic Light Scattering is a reliable method for accurately measuring the size of viruses like HIV and HAV in a non-invasive manner.
  • DLS offers a valuable alternative or complementary technique to electron microscopy for virus characterization.