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Related Experiment Video

Updated: Mar 19, 2026

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions
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Cell and Tissue Display: An Alternative Multipurpose Tool for Microscopy.

Nicholas Theodosakis1,2, Goran Micevic1,2, Marcus W Bosenberg1,2

  • 1Department of Pathology, Yale University, New Haven, Connecticut (NT, GM, MWB)

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|June 9, 2016
PubMed
Summary
This summary is machine-generated.

We developed Cell and Tissue Display (CTD), a novel method for embedding multiple tissue samples in agarose blocks. This technique improves cell distribution and enables customized multiplexing for various histologic applications.

Keywords:
Cell and Tissue Displayhistologic blockhistology

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Area of Science:

  • Histology
  • Biotechnology
  • Pathology

Background:

  • Histologic processing often involves embedding single tissue samples per block.
  • Multiplexing small tissue fragments or cell lines in a single block presents challenges in uniform distribution and processing.

Purpose of the Study:

  • To introduce a novel method, Cell and Tissue Display (CTD), for embedding multiple distinct tissue samples within a single agarose block.
  • To demonstrate the utility of CTD for uniform multiplexing of cell lines and small tissue fragments.

Main Methods:

  • Developed the Cell and Tissue Display (CTD) method for creating multi-compartment agarose blocks.
  • Embedded 16 or more different tissue samples within these custom agarose blocks.
  • Evaluated cell and tissue distribution within the CTD-generated blocks.

Main Results:

  • The CTD method allows for uniform multiplexing of cell lines and small tissue fragments in a single histologic block.
  • CTD blocks demonstrated improved distribution of individual cells, attributed to small, uniform agarose compartments.
  • The composition of CTD blocks is customizable for specific applications.

Conclusions:

  • Cell and Tissue Display (CTD) offers a versatile approach for histologic sample preparation.
  • CTD facilitates improved sectioning of small biopsies and multiplexing of diverse tissue types.
  • This method supports the generation of control slides for laboratory proficiency testing.