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Extending the linker region increases the activity of the Bacillus subtilis cellulase CelI15
Pingping Li1, Yang Zhou1, Qian Li1
1School of Life Science, Zhengzhou University, 100 Kexue Street, Zhengzhou, 450001, China.
Objectives:
To investigate the effect of the linker region (LR) on the enzymatic activity, stability, and flexibility of Bacillus subtilis cellulase CelI15, six mutants were constructed that contained increasing numbers of the LR.
Results:
The CelI15 mutant with three copies of the LR (approx. 57 amino acids) showed the highest activity, which was almost 20 % greater than that of wild type CelI15. The stability of the mutant enzymes increased as the copy number of the LR decreased. However, the substrate affinity of the mutant enzymes increased as the LR copy number increased, and the mutant with four copies of the LR exhibited the highest substrate affinity. Additionally, the flexibility of the CelI15 mutants increased as the LR copy number increased from zero to four copies, although it decreased sharply for the mutant with five copies of the LR.
Conclusion:
The activity of CelI15 was increased by increasing the LR copy number, which could be a potential way to improve its enzymatic properties.
