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Studying Neutrophil Migration In Vivo Using Adoptive Cell Transfer
Yoshishige Miyabe1, Nancy D Kim1, Chie Miyabe1
1Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, 13th Street, Charlestown, Boston, MA, 02129, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 9, 2016
Summary
Adoptive neutrophil transfer in the K/BxN arthritis model reveals key regulators of immune cell migration. This method defines how neutrophil receptors influence their entry into inflamed joints, aiding autoimmune disease research.
Area of Science:
- Immunology
- Cell Biology
- Rheumatology
Background:
- Immune cell migration is crucial in inflammatory diseases like rheumatoid arthritis (RA).
- Chemoattractants and their receptors control cell movement, with dysregulation linked to autoimmune conditions.
- The K/BxN serum transfer model mimics RA, highlighting neutrophils' role in joint inflammation.
Purpose of the Study:
- To define the role of neutrophil-specific receptors and mediators in joint recruitment.
- To utilize adoptive neutrophil transfer to study immune cell trafficking in vivo.
- To investigate the mechanisms of neutrophil entry into inflamed joints within the K/BxN model.
Main Methods:
- Adoptive transfer of neutrophils in the K/BxN serum transfer mouse model.
- Analysis of chemoattractant receptors, cytokines, and activation receptors on neutrophils.
- In vivo tracking of neutrophil migration to inflamed joints.
Main Results:
- Adoptive neutrophil transfer successfully identified critical regulators of neutrophil recruitment.
- Specific neutrophil receptors and mediators were found to significantly influence joint entry.
- The study elucidated the migratory behavior of neutrophils in an autoimmune arthritis setting.
Conclusions:
- Adoptive cell transfer is a powerful technique for studying immune cell migration in vivo.
- Understanding neutrophil trafficking is key to developing therapies for inflammatory arthritides.
- This approach provides insights into immune cell behavior in autoimmune disease models.
