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Related Experiment Video

Updated: Mar 19, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes
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CRISPR/Cas9: a breakthrough in generating mouse models for endocrinologists.

Suzy Markossian1, Frédéric Flamant2

  • 1Institut de Génomique Fonctionnelle de LyonUniversité de Lyon, CNRS, INRA, École Normale Supérieure de Lyon, Lyon Cedex 07, France.

Journal of Molecular Endocrinology
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CRISPR/Cas9 gene editing in mice offers powerful genome modification but faces challenges. Mosaicism and off-target mutations in founder mice are key limitations, though progress is being made to overcome these issues.

Keywords:
CRISPRCas9genome editingtransgenic mice

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Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • CRISPR/Cas9 technology has emerged as a powerful tool for genome editing.
  • Its application in mice is rapidly expanding the genetic research toolkit.
  • CRISPR/Cas9 enables precise, targeted modifications of the genome with high efficiency.

Purpose of the Study:

  • To highlight the capabilities of CRISPR/Cas9 for in ovo genome editing in mice.
  • To identify and discuss the primary limitations of current CRISPR/Cas9 applications in mouse embryos.
  • To suggest future directions for overcoming these challenges in genome editing research.

Main Methods:

  • In ovo CRISPR/Cas9 delivery into mouse embryos.
  • Analysis of founder mice for genetic modifications.
  • Assessment of mosaicism and off-target mutations.

Main Results:

  • CRISPR/Cas9 allows for highly efficient and targeted genome editing in mouse embryos.
  • Mosaicism is a frequent occurrence in founder mice generated via in ovo editing.
  • Evaluating off-target mutations poses a significant challenge, often requiring germline transmission analysis.

Conclusions:

  • CRISPR/Cas9 is a transformative technology for mouse genetics, offering unprecedented genome editing capabilities.
  • Current limitations in in ovo editing, specifically mosaicism and off-target mutation detection, require further research.
  • Ongoing advancements suggest that these challenges will likely be resolved in the near future, enhancing the utility of CRISPR/Cas9 in mouse models.