Expression Pattern of Matrix Metalloproteinase 20 (MMP20) in Human Tumors
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Updated: Mar 19, 2026
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
Published on: July 23, 2015
Dominik Kraus1, Jan Reckenbeil1, Sven Perner2
1Department of Prosthodontics, Preclinical Education and Material Science, University of Bonn, Bonn, Germany.
Background/Aim:
Matrix metalloproteinase 20 (MMP20) is a member of the family of matrix metalloproteinases. Under normal conditions the expression of MMP20 is restricted to ameloblasts and odontoblasts. In order to identify a possible expression of MMP20 under pathological conditions, we investigated three major human tumor entities, i.e. colon, breast and lung tumors, on the mRNA and protein level.
Materials And Methods:
Real-time RT-PCR and immunocytochemical analyses of established human tumor cell lines were employed for our study; immunohistochemical analysis was performed on both primary tumors and normal control tissues.
Results:
MMP20 was identified on both the mRNA and the protein level in breast MCF-7, colon HT-29, and lung A549 cell lines. MMP20 was also detected in primary tumor tissue by immunohistochemistry.
Conclusion:
MMP20 is a new potential candidate for tumor diagnosis or therapy.
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