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Updated: Mar 19, 2026
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
Published on: July 23, 2015
Expression Pattern of Matrix Metalloproteinase 20 (MMP20) in Human Tumors
Dominik Kraus1, Jan Reckenbeil1, Sven Perner2
1Department of Prosthodontics, Preclinical Education and Material Science, University of Bonn, Bonn, Germany.
Background/Aim:
Matrix metalloproteinase 20 (MMP20) is a member of the family of matrix metalloproteinases. Under normal conditions the expression of MMP20 is restricted to ameloblasts and odontoblasts. In order to identify a possible expression of MMP20 under pathological conditions, we investigated three major human tumor entities, i.e. colon, breast and lung tumors, on the mRNA and protein level.
Materials And Methods:
Real-time RT-PCR and immunocytochemical analyses of established human tumor cell lines were employed for our study; immunohistochemical analysis was performed on both primary tumors and normal control tissues.
Results:
MMP20 was identified on both the mRNA and the protein level in breast MCF-7, colon HT-29, and lung A549 cell lines. MMP20 was also detected in primary tumor tissue by immunohistochemistry.
Conclusion:
MMP20 is a new potential candidate for tumor diagnosis or therapy.
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