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Endometrial Adenocarcinoma: Analysis of Circulating Tumour Cells by RT-qPCR
Alexandra C Kölbl1, Rebekka Wellens1, Julian Koch1
1Department of Obstetrics and Gynecology, Ludwig-Maximilians University of Munich, Munich, Germany.
Background:
Endometrial adenocarcinoma is a frequently occurring cancer in women, accounting for 42,000 deaths every year. Despite treatment with standard therapy, occurrence of remote metastases and local recurrences is high. Through help of RT-qPCR minimal residual disease could be detected and characterized, facilitating therapeutic decision making.
Materials And Methods:
A number of marker genes were first tested in model systems and genes that performed best, were consequently used for the examination of 13 blood samples from endometrial carcinoma patients.
Results:
Cytokeratin 19 and MIG7 were chosen for the analysis in patient samples. Both genes were found up-regulated in small tumours and in one large tumour, but no statistical correlations could be revealed between expression levels of these two genes and tumour characteristics.
Conclusion:
There seems to be a coherence between gene expression and the stage of tumorigenesis, but the number of samples is still too small, to be able to obtain statistical significant differences.
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