Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ossification variants of the distal femoral condyle: longitudinal 3 T MRI evidence of progression to juvenile osteochondritis dissecans in asymptomatic siblings of patients with JOCD.

Skeletal radiology·2026

Same author

Magnetoelectric microrobots for spinal cord injury regeneration.

Nature materials·2026

Same author

The Efficacy of Bracing in Nonoperative Care of Medial Femoral Condyle Osteochondritis Dissecans: A Study From the Research in Osteochondritis Dissecans of the Knee (ROCK) Study Group.

The American journal of sports medicine·2026

Same author

Magnetoelectric Nanoparticle-Based Wireless Brain-Computer Interface: Underlying Physics and Projected Technology Pathway.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Magnetic continuum soft robot-driven precise delivery of prodrug nanoassemblies for gastric cancer chemo-immunotherapy.

Asian journal of pharmaceutical sciences·2026

Same author

Oxygen Isotope Fingerprints of Electron-Phonon Coupling in SrVO_{3} Films.

Physical review letters·2026

Search research articles

Related Experiment Video

Updated: Mar 19, 2026

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

An Atomic Force Microscope with Dual Actuation Capability for Biomolecular Experiments.

Semih Sevim¹, Naveen Shamsudhin², Sevil Ozer¹

¹Department of Electrical and Electronics Engineering, Bogazici University, Bebek 34342 Istanbul, Turkey.

Scientific Reports

|June 9, 2016

Summary

This summary is machine-generated.

We developed a modular atomic force microscope (AFM) for biomolecular studies. This design offers high force actuation and precise force resolution, enabling experiments like molecular pulling and unbinding studies.

More Related Videos

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Published on: July 5, 2022

Related Experiment Videos

Last Updated: Mar 19, 2026

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Published on: July 5, 2022

Area of Science:

Biophysics
Nanotechnology
Materials Science

Background:

Atomic Force Microscopy (AFM) is crucial for nanoscale investigations.
Existing AFM designs can be complex and costly for specific biomolecular applications.

Purpose of the Study:

To report a novel modular AFM design optimized for biomolecular experiments.
To integrate high-force actuation capabilities for advanced force measurements.

Main Methods:

A modular AFM head was constructed using readily available components and jetted-polymers for rapid assembly.
Deflection-based optics and a piezotube actuator were incorporated.
A FeCo-tipped electromagnet was utilized for high-force cantilever actuation (up to 0.55 T).
Magnetic field calibration was performed using a micro-hall sensor and finite element simulations.

Main Results:

Achieved an integrated force resolution of 1.82 pN in air and 2.98 pN in DI water at a 1 kHz bandwidth.
Demonstrated electromagnetic and piezoelectric actuation for molecular unbinding experiments.
Successfully performed biotin-streptavidin complex unbinding studies.

Conclusions:

The modular AFM design facilitates rapid manufacturing and scalability.
The integrated high-force actuation and precise force resolution enable diverse biomolecular force measurements.
This AFM system is suitable for various modes like molecular pulling and force-clamp experiments.