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Related Concept Videos

Immunogold Electron Microscopy01:20

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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Related Experiment Video

Updated: Mar 19, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

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Embedding Raman Tags between Au Nanostar@Nanoshell for Multiplex Immunosensing.

Ting Yang1, Jiang Jiang2

  • 1i-Lab and Division of Nanobiomedicine, CAS Key Laboratory of Nano-Bio Interface, CAS Center for Excellence in Nanoscience, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.

Small (Weinheim an Der Bergstrasse, Germany)
|June 9, 2016
PubMed
Summary

New gold nanostar and nanoshell structures act as highly sensitive Raman tags for biosensing. These novel composites demonstrate superior surface-enhanced Raman scattering, enabling sensitive and multiplexed immunoassays.

Keywords:
Au nanostarsSERSembedded dyesimmunosensingmultiplex

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Surface-enhanced Raman scattering (SERS) is a powerful technique for sensitive molecular detection.
  • Gold nanoparticles are commonly used for SERS applications, but their efficiency can be limited.
  • Developing novel nanostructures can enhance SERS performance.

Purpose of the Study:

  • To develop novel Raman tags using gold nanostar (AuNS) and gold nanoshell composites.
  • To investigate the SERS properties of these new structures.
  • To evaluate their performance in immunoassay applications.

Main Methods:

  • Synthesis of AuNS@tag@shell structures with reporter molecules.
  • Characterization of SERS intensity compared to gold nanoparticle composites.
  • Development and testing of an immunoassay using the developed structures.

Main Results:

  • The AuNS@tag@shell structures exhibited significantly enhanced SERS intensity.
  • Immunoassays demonstrated high specificity with a sensitivity limit of 0.1 pg mL⁻¹.
  • The developed structures enabled multiplex detection capabilities.

Conclusions:

  • Novel gold nanostar and nanoshell composites serve as highly effective Raman tags.
  • These structures offer superior SERS enhancement for biosensing.
  • The developed immunoassay platform shows great promise for sensitive and multiplexed diagnostics.