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Characterization of a novel MICA allele, MICA*012:05, by cloning and sequencing
1Immunogenetics Research Group, Department of Immunology, College of Basic Medical Sciences, Central South University, Changsha, China.
Abstract:
A new MICA allelic variant, MICA*012:05, has been identified in a Chinese Mongolian population. Following polymerase chain reaction-sequence-based typing (PCR-SBT), this new allele was further confirmed by cloning and sequencing. MICA*012:05 was linked to an HLA-A*24-C*01-B*55:02-DRB1*09 haplotype. MICA*012:05 differs from MICA*012:01 by a single synonymous C to T substitution at nucleotide position 269 in exon 3.
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