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Related Experiment Video

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Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease
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Induced Sputum Analysis in Subjects With Systemic Sclerosis.

Irena Litinsky1, Elizabeth Fireman2, Daphna Paran3

  • 1Department of Rheumatology litinskyira@yahoo.com.

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|June 9, 2016
PubMed
Summary

Induced sputum analysis reveals distinct cellular patterns in systemic sclerosis patients, correlating with disease severity and lung function. These findings offer a noninvasive method to assess systemic sclerosis lung involvement.

Keywords:
induced sputuminflammationinterstitial lung diseasesystemic sclerosis

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Area of Science:

  • Pulmonary Medicine
  • Immunology
  • Rheumatology

Background:

  • Induced sputum analysis is a validated noninvasive method for assessing airway cellular and soluble materials.
  • Its utility in systemic sclerosis (SSc) and correlation with disease manifestations require further investigation.

Purpose of the Study:

  • To evaluate the diagnostic utility of induced sputum analysis in SSc patients.
  • To determine correlations between induced sputum findings and clinical/pulmonary function parameters in SSc.

Main Methods:

  • A cohort of 20 SSc patients and 16 healthy controls underwent pulmonary function testing (PFT) and induced sputum cytology.
  • Analysis included cell counts, CD markers, and correlations with clinical scores (e.g., modified Rodnan skin score) and PFT parameters.

Main Results:

  • SSc patients exhibited higher neutrophil and lower lymphocyte counts in induced sputum compared to controls.
  • Significant correlations were observed between skin scores, CD markers, and sputum cell differentials.
  • Neutrophil counts inversely correlated with lung volumes (TLC, FVC), while lymphocyte counts correlated positively with TLC.

Conclusions:

  • Induced sputum analysis reveals altered cellular profiles in SSc patients, including those with restrictive lung disease.
  • These cellular changes correlate significantly with clinical and PFT parameters, suggesting its potential as a biomarker for SSc lung involvement.