Related Experiment Video
Updated: Mar 19, 2026
07:34
Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
Published on: November 30, 2015
9.9K
Whole transcriptome data analysis of zebrafish mutants affecting muscle development
Olivier Armant1, Victor Gourain1, Christelle Etard1
1Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Campus Nord, PO box, Karlsruhe, Germany.
Data in Brief
|June 9, 2016
Summary
Skeletal muscle development involves complex myofibril formation. This study reveals that impaired myosin folding in zebrafish mutants triggers a distinct gene expression pattern, differing from general muscle stress responses.
Area of Science:
- Muscle Biology
- Developmental Biology
- Genomics
Background:
- Skeletal muscle myofibril formation is crucial for function.
- Perturbations in this process can lead to muscular dystrophy.
- Understanding the genetic regulation of muscle development is essential.
Purpose of the Study:
- To provide a mRNA sequencing dataset for zebrafish mutants affecting muscle organization.
- To investigate the transcriptional response to impaired myosin folding.
- To differentiate gene expression profiles in specific muscle defects versus general stress.
Main Methods:
- Transcriptome analysis (mRNAseq) of three zebrafish mutants: unc45b-/- (myosin folding chaperone), hsp90aa1.1-/- (heat shock protein), and ache-/- (acetylcholine esterase).
- Experiments conducted at 72 hours post-fertilization (hpf), with additional time points (24, 48 hpf) for unc45b-/-.
- Differential gene expression analysis using hierarchical clustering on 20 samples.
Main Results:
- Identification of unique transcriptional programs in skeletal muscles of mutants with myosin folding defects.
- Comparison of gene expression profiles between myosin folding failure and general muscle stress.
- Dataset provides insights into the specific molecular pathways affected by mutations in unc45b, hsp90aa1.1, and ache.
Conclusions:
- Failure in myosin folding initiates a unique skeletal muscle transcriptional program.
- This program is distinct from the response observed in general muscle stress.
- The study provides valuable transcriptomic data for understanding muscle development and disease.
