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Updated: Mar 19, 2026

Methodology for Sputum Induction and Laboratory Processing
Published on: December 17, 2017
Educational intervention for collecting sputum for tuberculosis: a quasi-experimental study
Amélia Nunes Sicsú1, Julia Ignez Salem2, Luciana Botinelly Mendonça Fujimoto3
1Doctoral student, Escola de Enfermagem de Ribeirão Preto, Universidade de São Paulo, PAHO/WHO Collaborating Centre for Nursing Research Development, Ribeirão Preto, SP, Brazil. Assistant Professor, Escola Superior de Ciências da Saúde, Universidade do Estado do Amazonas, Manaus, AM, Brasil. Scholarship holder from Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM), Brazil. , Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto SP , Brazil.
Nursing guidance significantly improved sputum sample quality for tuberculosis diagnosis. This educational intervention enhanced sample purity, volume, and diagnostic accuracy in patients with suspected pulmonary tuberculosis.
Area of Science:
- Pulmonary Medicine
- Infectious Diseases
- Nursing Education
Background:
- Sputum sample quality is crucial for accurate tuberculosis diagnosis.
- Inadequate sample collection can lead to false-negative results and delayed treatment.
- Standardized nursing guidance can potentially improve sputum collection efficacy.
Purpose of the Study:
- To assess the impact of nursing guidance on sputum sample quality.
- To evaluate changes in sputum characteristics (purulence, volume) post-intervention.
- To determine the effect of improved sample quality on tuberculosis diagnostic rates.
Main Methods:
- A quasi-experimental, single-group, before-and-after study design was employed.
- 138 patients with suspected pulmonary tuberculosis received individualized sputum collection guidance.
- Intervention included adherence to Ministry of Health guidelines and educational materials.
Main Results:
- A significant increase in sputum samples containing purulent particles was observed.
- Sputum sample volume significantly increased, exceeding 5 mL post-intervention.
- The rate of tuberculosis diagnosis among patients increased following the educational intervention.
Conclusions:
- Nursing guidance demonstrably improves sputum sample quality for bacilloscopic examination.
- Enhanced sample aspect and volume contribute to more effective tuberculosis diagnosis.
- Educational interventions are vital for optimizing diagnostic procedures in suspected pulmonary tuberculosis cases.
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