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Related Experiment Videos

Biodegradation of crude oils.

K Bosecker, M Teschner, H Wehner

    Schriftenreihe Des Vereins Fur Wasser-, Boden- Und Lufthygiene
    |January 1, 1989
    PubMed
    Summary

    Microorganisms in petroleum can biodegrade crude oils, altering physical properties and producing heavy oil. This in-situ biodegradation process mimics natural oil transformation, impacting the petroleum industry.

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    Area of Science:

    • Geochemistry
    • Microbiology
    • Petroleum Science

    Background:

    • Petroleum reservoirs harbor diverse microorganisms.
    • These microbes possess the capability to degrade crude oil components.

    Purpose of the Study:

    • To investigate the biodegradation of crude oils by isolated microorganisms.
    • To optimize conditions for microbial degradation and analyze resulting oil properties.

    Main Methods:

    • Isolation and inoculation of microorganisms from petroleum samples.
    • Chromatographic techniques to monitor oil degradation.
    • Optimization of reaction parameters (pH, nutrients, oxygen).

    Main Results:

    • Isolated microorganisms (yeast, bacteria) effectively degraded n-alkanes.
    • Biodegradation altered oil properties, increasing density and viscosity towards heavy oil.
    • Selective degradation of medium-chain n-alkanes observed under limiting conditions.
    • Aromatic hydrocarbons and biomarkers remained undegraded.

    Conclusions:

    • In-situ biodegradation of conventional oils is a significant factor in heavy oil formation.
    • Microbial degradation in vitro closely resembles natural biodegradation processes.
    • Optimized microbial consortia show potential for enhanced oil recovery or remediation.

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