Quantification of 18 amino acids in human plasma: application in renal transplant patient plasma by targeted
Qinghua Wang1,2, Yan Wen1, Tianyi Xia1
1Department of Pharmacy, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China.
Aim:
Quantification of amino acids in human plasma has become an important and essential analysis parameter in life science. In this paper, we developed a targeted UHPLC-MS/MS method for 18 amino acids in the renal transplant patients.
Methods & Results:
Plasma in small volume (150 μl) was pretreated by a one-step protein precipitant extraction for analysis. Detection was executed by MS/MS in the MRM mode. Assays were validated according to current bioanalytical guidelines, with good linearity (R > 0.99), intraday and interday precision (CV < 11.6%, RE ≤ ± 14.8%), extraction recovery (between 77.4 and 117.6%), matrix effect (73.3-118.0%) and stability (RE≤ ±14.7%).
Conclusion:
The method was successfully applicable for amino acid analysis in the renal transplant patient.
