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Updated: Mar 19, 2026
An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
Published on: December 3, 2020
Development and validation of a UPLC-MS/MS method for the simultaneous determination of paritaprevir and ritonavir in
Andrew J Ocque1, Colleen E Hagler1, Robin Difrancesco1
1Translational Pharmacology Research Core, Department of Pharmacy Practice, School of Pharmacy & Pharmaceutical Sciences, New York State Center of Excellence in Bioinformatics & Life Sciences, University at Buffalo, Buffalo, NY, USA.
Aim:
Determination of paritaprevir and ritonavir in rat liver tissue samples.
Results:
We successfully validated a UPLC-MS/MS method to measure paritaprevir and ritonavir in rat liver using deuterated internal standards (d8-paritapervir and d6-ritonavir). The method is linear from 20 to 20,000 and 5 to 10,000 pg on column for paritaprevir and ritonavir, respectively, and is normalized per milligram tissue. Interday and intraday variability ranged from 0.591 to 5.33% and accuracy ranged from -6.68 to 10.1% for quality control samples. The method was then applied to the measurement of paritaprevir and ritonavir in rat liver tissue samples from a pilot study.
Conclusion:
The validated method is suitable for measurement of paritaprevir and ritonavir within rat liver tissue samples for PK studies.
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